Showing posts with label cell biology. Show all posts
Showing posts with label cell biology. Show all posts

Sunday, February 28, 2010

What Are Scientists Made Of?


Last Thursday, the Duke Career Center, the Duke Institute for Genome Sciences & and Policy (IGSP) and Women in Science and Engineering (WISE) sponsored a screening of the documentary “Naturally Obsessed: The Making of a Scientist,” the story of three PHD candidates working to establish their careers in a Columbia University lab. The students are working to isolate proteins and determine their structures using X-ray crystallography, a difficult technique that requires a lot of luck.

The documentary, filmed over the course of three years, captures the heartbreaking failure that can follow months of work, as well as the surprising triumph that can result from a breakthrough. Each of the three students has their own reason for being there, and each approach their research in a different way. (Click here to read a review)

At the beginning of the movie, lab director Larry Shapiro somewhat controversially says that “one of the best things you can do as a scientist is suffer from Obsessive Compulsive Disorder (OCD). You become obsessed with a problem and can’t stop working on it until you find your answer.”

However, Dr. Rochelle Schwartz-Bloom, director of Duke’s Center for Science Education, argues that “you don’t have to be OCD or obsessed to love what you’re doing, you just have to have passion for it.”

Schwartz-Bloom was one of six panelists who shared their thoughts with the audience after the movie.

Grad student Vincent Chen was impressed with the film’s accuracy “in terms of the ups and downs in science. You have a lot of failures, and very few successes.”

Grad student Cynthia Tedore disagreed. “I didn’t see the repeated failure as accurate from my point of view. In any field, it’s bad to pursue one project with a small chance of succeeding. It’s better to pursue several.”

A member of the audience commented that, based on her experience in an X-ray crystallography lab, she thought the filmmakers did a good job. “I was touched by everything that happened. There are upsides and downsides. X-ray crystallography is very different from other fields, so [the movie] might not reflect what happens in other labs.”

Schwartz-Bloom said that the movie made her nervous with its emphasis on hard labor with a small chance of success. “A lot of kids today don’t go into science because they think it’s ‘too hard.’ Even people with some passion for it decide that they don’t want to do it.” She worried that the movie would only encourage this sentiment.

Fortunately, not everyone was so discouraged. My bio major friend told me that, despite the grim portrayal, she had not been dissuaded from her goal to be a scientist.

At the end of the discussion, the panelists offered their advice for aspiring researchers like my friend.

“Sometimes it is important to know when to quit, when a problem is impossible to solve. Otherwise, you can trash your entire career.” -- post-doc Rebekah Fleming

“Be a sponge. Take in everything you can, because you don’t know when you’ll need it in the future.” -- Schwartz-Bloom

“Don’t be afraid to find out what your weaknesses are, and how to fix them.” -- Tedore

“Science should be fun. If you’re doing it for your boyfriend or for your parents, you can do it for a while, but eventually it’ll break your heart.” -- panel moderator, professor Mohamed Noor of the Biology Department

Tuesday, February 2, 2010

How Do Rodents Make Decisions? A Nobel Laureate Speaks

Ion channels, the small tunnels that allow charged particles to flow through the cell membrane, play a significant role in various physiological functions. For neurobiologists, measuring the electric current associated with these ion channels proved to be a hard task, as a vast amount of information was lost due to noise.


The technique that was developed to overcome the loss of electric signals is called the patch clamp, and is one of the most widely used tools to study physiological processes on a molecular level today. One of the co-developers of this technique and a Nobel laureate, Dr. Bert Sakmann, was here on Friday to talk about his research at a seminar organized by the Duke Institute for Brain Sciences.

'We studied the neural connections in rodents using the Whisker model", Sakmann said to a packed auditorium.


The Whisker model was a whisker-dependent learning task in which the rodents had to make the decision whether to cross a small gap or not. "We wanted to know how many (neuronal) columns (of the brain were) involved in the decision-making process."

Through these experiments, Dr. Sakmann and fellow Nobel Laureate Dr. Erwin Neher identified certain cortical circuits that were activated during this decision-making process. "We measured the latency between stimulus and activation, and found there is a very precise and small latency."

They further discovered that decision-making is possible using a single column in the cortex. To measure the electric current associated within such single cells, they developed the patch clamp technique.

The patch clamp enabled the study of single ion channels and helped gain insight into the role of these channels in hormone regulation, heart diseases, epilepsy, and diabetes among others. A small video demonstrating the technique can be found below.

Sakmann and Neher received the Nobel Prize for Medicine in 1991 for their work.